Lauren Gray
Determining the source of excess phosphatase in hypothetical patient
Abstract
Enzymes are diverse, biological catalysts that induce and regulate chemical reactions. Assays can be performed to determine the specific biochemical properties of enzymes that regulate the same reactions but have slightly different amino acid sequences. In stressed biological systems, enzymatic activities fluctuate and the source of a medical condition can be linked to abnormal concentrations of the specific isozyme that functions in an area of the body. The purpose of this experiment was to determine the source of excess phosphatase in a patient suffering from one of six potential conditions. To distinguish between bacterial, fungal, prostate-specific, hepatic, renal and intestinal phosphatases three assays were performed. The unknown phosphatase's optimal pH and response to potential inhibitors 10mM EDTA and 1mM Levamisol were determined and compared to given information on the......

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Title: Determining The Source Of Excess Phosphatase In Hypothetical Patient
Approximate Word Count: 1294
Approximate Pages: 6 (250 words per double-spaced page)

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