Exercise 5

Introduction:

Many different species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species, one can look at the metabolic differences (fermentation), as well as the environmental condition differences (temperature, pH, oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media can be used to culture and identify bacteria.
Some bacteria require specific nutrients and conditions, while others can make due with whatever the environment has available. Some bacteria lack the enzymes to break down a complex carbon source, while others can break it down easily. Some bacteria require oxygen while others can not tolerate it. Some bacteria can live in harsh environments such as salt, while others will not grow in the harsh environment.
There are four different media......

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Title: Culturing: Media Selection And Inoculation Technique
Approximate Word Count: 708
Approximate Pages: 3 (250 words per double-spaced page)

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