Abstract
The purpose of this experiment was to study the transfer of genetic information on plasmid F’lac by using Escherichia coli. Plasmid transfer was measured by using two different methods. The first one was by using selection and contraselection with three antibiotics: streptomycin(which was replaced by naladixic acid for the second part of the experiment),ampicillin and kanamycin and the second one by using a colour indicator ( X-gal). As significant results, the percentage of transfer for F’lac was higher than the percentage for transposition. Also, the experiment demonstrated that E.coli can quickly acquire resistance to several different antibiotics through the transfer of the F’lac plasmid. It was concluded that significant changes on the genetic makeup can be achieved through transposition and conjugative plasmids in a short amount of time, which can have severe implication on the effectiveness of antibiotics for bacterial diseases.......

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Title: Conjugation And Transposition In Escherichia Coli
Approximate Word Count: 2519
Approximate Pages: 11 (250 words per double-spaced page)

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