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Determining the Optimal Temperature and PH of Barley Amylase
Abstract
The purpose of this experiment was to find the optimal temperature and pH of barley alpha-amylase. I hypothesize that the optimal temperature would be 55 degrees Celsius and the optimal pH would be 5.5. In this experiment, the starch is used as a substrate to examine the optimum temperature and pH for the reaction of alpha amylase. It is known that the measuring of disappearance (absorbance) of the substrate starch with iodine using spectrophotometer will show the concentration of the substrate which will also reflect on the reaction rate. Once the reaction rates are figured out, the optimal temperature and pH can be determined. The result concluded that the optimal temperature was at 50 degrees Celsius and the optimal pH was at 5.0. I have evaluated that my hypothesis was not supported through this experiment however, it has been clarified that the optimal temperature and pH of barley alpha-amylase turns......
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Title: Determining The Optimal Temperature And Ph Of Barley Amylase
Approximate Word Count: 1320
Approximate Pages: 6 (250 words per double-spaced page)
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