AP Lab 2: enzyme catalysis report
Purpose:
To observe the conversion of hydrogen peroxide to water and oxygen gas by the enzyme catalase, measure the amount of oxygen generated, and calculate the rate of the enzyme-catalyzed reaction.
Hypothesis:
If the time of enzyme reacts with the solution longer, then the rate of biochemical reaction would decreases.
Procedure:
See attached flow chart
Data:

Table 2.1 catalase reaction times assay
time(S) A500 H2O2 (M) avg a500 avg H2O2(M)
0.00 1.24 2.73E-03 1.36 2.99E-03
30.00 1.08 2.38E-03 1.28 2.82E-03
60.00 1.15 2.53E-03 1.22 2.68E-03
90.00 1.04 2.29E-03 1.14 2.51E-03
120.00 1.03 2.26E-03 1.08 2.38E-03
150.00 0.92 2.02E-03 1.01 2.22E-03
180.00 0.90 1.08E-03 0.88 1.94E-03
(1.24/5000)x11=0.00273 (1.36/5000)x11=0.00299
(1.08/5000)x11=0.00238 (1.28/5000)x11=0.00282
(1.15/5000)x11=0.00253 (1.22/5000)x11=0.00268
(1.04/5000)x11=0.00229......

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Title: Ap Lab 2
Approximate Word Count: 745
Approximate Pages: 3 (250 words per double-spaced page)

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