Implementing: -

1. First, I gathered the necessary equipment:
§ Thermostatically controlled water baths
§ Ice
§ Thermometer
§ Stop watch
§ Test tubes
§ Photographic film (b/w)
§ Syringe
§ Test tube rack
§ Ruler
§ Scissors
§ O.1% trypsin made in pH7 buffer solution
§ Mounted needle

2. I then decided on the range of results and temperatures I was to investigate. I decided to observe 20º, 30º, 40º, 50º, 60º, 70º and 80º.
3. Next, I label each test tube with the temperature to be investigated and then added 2.5cm3 of Trypsin solution.
4. I ensured that I cut the necessary pieces of film using a ruler and a pair of scissors. I cut pieces that were 2cm in length, and 1.6cm in width each.
5. The only water baths of my choice that were running were at 30º and 60º. I therefore had to use the Bunsen burner etc. for the rest of the temperatures (excluding 20º). I kept each test tube heated for 5 minutes.
6. I added the photographic film pieces just......

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Title: Biology Coursework: The Effect Of Trypsin On Gelatine.
Approximate Word Count: 681
Approximate Pages: 3 (250 words per double-spaced page)

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