SANGER METHOD OF DNA SEQUENCING

DNA sequence determination is based on the high resolution separation of

nucleotides on polyacarylamide or an algarose gel. One method used in determining this

sequence is called the Sanger procedure. The components used are: a polyacarylamide or

an algarose gel, DNA polymerase, a primer (a short sequence), template DNA (the DNA

to be sequenced), deoxynucleotide triphosphate, and dideoxynucleitide phosphate. With

all of these, double stranded DNA is made continuously until all the triphosphates are

used up.
The deoxynucleotides, are deoxyadenine triphosphate, deoxyguanine triphosphate,

deoxycytocine triphosphate, and deoxythymine tryphosphate. They are the same as

nucleotides with the exception being that they contain hydrogen on the 3' carbon instead

of an OH. Therefore, when these deoxynucleitides are integrated into a sequence, they

prevent the addition of further nucleotides because......

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Title: Sanger Method
Approximate Word Count: 594
Approximate Pages: 3 (250 words per double-spaced page)

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