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SANGER METHOD OF DNA SEQUENCING

DNA sequence determination is based on the high resolution separation of

nucleotides on polyacarylamide or an algarose gel. One method used in determining this

sequence is called the Sanger procedure. The components used are: a polyacarylamide or

an algarose gel, DNA polymerase, a primer (a short sequence), template DNA (the DNA

to be sequenced), deoxynucleotide triphosphate, and dideoxynucleitide phosphate. With

all of these, double stranded DNA is made continuously until all the triphosphates are

used up.
The deoxynucleotides, are deoxyadenine triphosphate, deoxyguanine triphosphate,

deoxycytocine triphosphate, and deoxythymine tryphosphate. They are the same as

nucleotides with the exception being that they contain hydrogen on the 3' carbon instead

of an OH. Therefore, when these deoxynucleitides are integrated into a sequence, they

prevent the addition of further nucleotides because...

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Title: SANGER METHOD
Approximate word count: 594
Approximate pages: 3 (250 words per double-spaced page)

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1. Sanger Method
   

   SANGER METHOD. SANGER METHOD OF DNA ... or an algarose gel. One method used in
   determining this sequence is called the Sanger procedure. ...

2. Dna
   

   ... Automated Sequencing With the many advancements in technology that we have achieved
   since 1974, it is no surprise that the Sanger method has become outdated. ...

3. Nobel Peace Prize
   

   ... In 1975, he developed the chain termination method of DNA sequencing, also
   known as the Dideoxy termination method or the Sanger method. ...

4. Human Development
   

   ... theory and methods (Le Richie and Tanner 1998 p28; Sanger 1996 p11 ... In applying
   naturalistic participant observation theory/method I experience the advantage of ...

5. An Outline Of The General Scentific Insights That Microbial Genome ...
   

   ... Microbial genome sequencing may be first dated back to the work of Frederick Sanger,
   who in 1975 developed the “chain termination method”, or Dideoxy ...